扫一扫，添加微信号
Phosphotyrosine Antibody, Biotin
|
Catalog# roduct Description |
ICP9901 The affinity purified rabbit anti-phospho-tyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays. |
|
Species
|
Rabbit
|
|
Formulation
|
250 µg/mL in PBS, 50% glycerol, 0.01% NaN3
|
|
Immunogen
|
Iodoacetylphosphotyamine-KLH conjugates
|
|
Purification
|
The antibody was affinity purified with phospho-tyrosine immobilized on agarose.
|
|
Specificity
|
The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
|
|
Applications
|
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IP (5 µg/mg protein); IHC (10 µg/mL)
|
|
Scientific Description
|
Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine, which is considered a key step in the regulation of enzymatic activities such as signal transduction.
|
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
|
|
Product Specific References |
|
