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Catalog# Product Description |
ICP9821 The affinity purified rabbit anti-phospho-threonine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of threonine-phosphorylated proteins and peptides. |
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, pH 7, with 50% glycerol
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Immunogen
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Phospho-threonine-KLH conjugates and phosvitin
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Purification
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The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
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Specificity
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The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.
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Applications
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ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
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Scientific Description
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Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to form phospho-threonine, which enters various biosynthetic pathways in the body.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.
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Product Specific References |
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