扫一扫，添加微信号
|
Catalog# Product Description |
ICP9807 Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose. |
|
Lane1: Untreated kinase
Lane 2: Kinase treated with phosphatase PP1G
Lane 3: MW marker time course
 Western blot analysis of mouse extract spleen with ICP9807
A: Extract of spleen from mouse treated with sodium vanadate
B: Extract of spleen from control mouse
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 µg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3 |
||
|
Immunogen
|
Phospho-threonine KLH conjugates and phosvitin mixture
|
||
|
Purification
|
The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
|
||
|
Specificity
|
The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.
|
||
|
Species Tested
|
Human and mouse
|
||
|
Applications
|
ELISA; WB; IP
|
||
|
Scientific Description
|
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
|
||
|
Product Specific References |
|
