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Phosphotyrosine Antibody, HRP
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Catalog# Product Description |
ICP9902 The affinity purified rabbit polyclonal anti-phospho-tyrosine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. |
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Immunoprecipitated samples with anti-phospho-tyrosine agarose (ICP9804)
Left: Mouse spleen lysate treated with complete adjuvant Right: Mouse spleen lysate without any treatment Detection: Phospho-tyrosine antibody, HRP (ICP9902)
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Species
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Rabbit
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Formulation
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250 µg/mL in HRP stabilizing buffer with 50% glycerol
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Immunogen
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Iodoacetylphosphotyramine-KLH conjugates
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Purification
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The antibody was affinity purified with phospho-tyrosine immobilized on agarose, followed by immune affinity absorption with tyrosine on agarose.
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Conjugation
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Reductive amination; HRP/Ab ratio - 10:1
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Specificity
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The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
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Applications
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ELISA (1:1000); WB (1:1000)
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Scientific Description
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Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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Product Specific References |
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