JNK Antibody, HRP

Catalog#

Product Description

ICP6031

These antibodies were tested with human and mouse cell lysates and mouse tissue (brain) extracts to detect Jun NH₂-terminal kinases (JNKs). JNK has a molecular weight ranging from 38-56 kDa, which is masked up by the IP IgG bands. Tests using the HRP conjugated anti-JNK do not require secondary Ab, eliminating interference from IgG bands.

Western blot analysis of mouse brain extract with ICP6030

A: Extract from control hypoxia mouse brain

B: Extract from control mouse

Species

Rabbit

Formulation

250 µg/mL in HRP stabilizing buffer with 50% glycerol

Immunogen

Human JNK1 (acetylated KLH conjugates)

Purification

The antibody was purified by peptide-affinity chromatography.

Conjugation

Reductive amination; HRP/Ab molar ratio - 10:1

Specificity

The recognition sequence in human and mouse is identical to JNK3, JNK2, and JNK1.

Applications

ELISA (1:1000); WB (1:1000)

Scientific Description

Jun NH2-terminal kinase (JNK) is a member of the mitogen-activatedprotein kinase family, which plays a central role in the regulationof cellular differentiation and proliferation. It also participatesin the regulation of IL-2 and IL-2 receptor gene expression. Over-activation of the c-JNK induces cell death.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.

Product Specific References

1. Proc Natl Acad Sci U S A. 1997. 94(10): 5119–5124.

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JNK Antibody, HRP

Catalog# Product Description	ICP6031 These antibodies were tested with human and mouse cell lysates and mouse tissue (brain) extracts to detect Jun NH₂-terminal kinases (JNKs). JNK has a molecular weight ranging from 38-56 kDa, which is masked up by the IP IgG bands. Tests using the HRP conjugated anti-JNK do not require secondary Ab, eliminating interference from IgG bands.	Western blot analysis of mouse brain extract with ICP6030 A: Extract from control hypoxia mouse brain B: Extract from control mouse
Species	Rabbit
Formulation	250 µg/mL in HRP stabilizing buffer with 50% glycerol
Immunogen	Human JNK1 (acetylated KLH conjugates)
Purification	The antibody was purified by peptide-affinity chromatography.
Conjugation	Reductive amination; HRP/Ab molar ratio - 10:1
Specificity	The recognition sequence in human and mouse is identical to JNK3, JNK2, and JNK1.
Applications	ELISA (1:1000); WB (1:1000)
Scientific Description	Jun NH2-terminal kinase (JNK) is a member of the mitogen-activatedprotein kinase family, which plays a central role in the regulationof cellular differentiation and proliferation. It also participatesin the regulation of IL-2 and IL-2 receptor gene expression. Over-activation of the c-JNK induces cell death.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
Product Specific References	1. Proc Natl Acad Sci U S A. 1997. 94(10): 5119–5124.