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Phospho-AKT (Ser473) Antibody
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Catalog# Product Description |
ICP0453 PKB alpha (AKT1) is a member of the PKB family, which is responsive to cell activation. The anti-PKB alpha antibodies were raised with a peptide based on the sequence of the human PKB C-terminal end and affinity purified with the same peptide on agarose. The affinity purified antibodies are useful for detection and isolation of PKB alpha from cell lysates. |
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A, B : Western blot analysis of FAB treated cell lysate with anti-pPKB (Ser473). ICP0453 and a monoclonal anti-pPKB (Ser473) from other supplier.
C: 5 ng/mL of FAB (anti-receptor) treatment with 10s D: Control - lymphocytes with no treatment
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Human PKB C-terminal peptide that is based on human PKB-KLH conjugates
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Purification
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The antibody was affinity purified with PKB phospho-peptide on agarose and affinity adsorption with non-phospho-peptide of the same sequence.
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Specificity
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Recognizes phosphorylated PKB on serine 473. There are no cross reactions with the non-phosphorylated PKB.
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Applications
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ELISA (0.25 µg/mL); WB (1 µg/mL); IP (10 µg/2 mg crude protein)
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Scientific Description
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AKT is also known as protein kinase B (PKB). It is an important molecule in mammalian cellular signalling. It inhibits apoptotic processes and is involved in cellular survival pathways. It also induces protein synthesis pathways.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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Product Specific References
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1. J. Clin. Invest. 2003. 112: 197-208. doi:10.1172/JCI16885
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