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Catalog# Product Description |
ICP9803 Affinity purified goat anti-rabbit IgG antibody is conjugated to horse radish peroxidase (HRP). The conjugate is useful for detection, identification, quantization and localization of rabbit antibodies. |
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Western blot analysis of rabbit anti-acetylated lysine with goat anti-rabbit IgG's HRP (ICP9803) as the secondary antibody.
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Species
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Goat
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Formulation
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1 mg/mL in HRP stabilizing buffer
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Immunogen
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Affinity purified rabbit immunoglobulins
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Purification
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The antibody was affinity purified with rabbit immunoglobulin on agarose followed by absorption with human gamma globulins and HSA.
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Specificity
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The antibody recognizes rabbit IgG and IgM. The antibody has not been tested for IgA and IgE recognition. There are no cross-reactions with human or mouse IgG.
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Conjugation
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Reductive amination; HRP/Goat Anti-Rabbit ratio - 10:1
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Applications
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ELISA (1:20,000); WB (1:10,000)
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Scientific Description
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IgG is a monomeric immunoglobulin. It is composed of two heavy chains (γ) and two light chains. Each IgG has two antigen binding sites that bind with many pathogens including viruses, bacteria and fungi. It also assists in phagocytosis and the neutralization of bacterial toxins.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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References
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1. Junqueira, L. C. at al. Basic Histology. 2003, McGraw-Hill.
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