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Catalog# Product Description |
ICP9601 The affinity purified anti-chicken IgY antibody is conjugated to horseradish peroxidase (HRP). The conjugate is useful for detection, identification, quantification and localization of chicken IgY, including the chicken egg yolk antibodies. |
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Species
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Goat
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Formulation
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1 mg/mL in stabilizing buffer with 50% glycerol
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Immunogen
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Affinity purified chicken IgY
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Purification
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Affinity purified with chicken IgY-agarose. Absorbed with human IgG-agarose and HSA-agarose.
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Specificity
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Specific to chicken IgY
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Conjugation
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Reductive amination. HRP: Antibody ratio = 2:1
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Applications
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ELISA (0.25 - 1.0 µg/mL), WB (0.25 - 1.0 µg/mL)
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Scientific Description
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In chickens, immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.
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