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Catalog# Product Description |
ICP0802 This antibody was raised from rabbits that were immunized with NG-mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are conjugated to peroxidase (HRP) and are useful for detection, isolation and localization of proteins with NG-methylated arginine residues. |
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Antigen Structure
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Species
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Rabbit
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Formulation
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250 µg/mL in stabilizing buffer with 50% glycerol
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Immunogen
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N5-mono-methyl arginine-KLH conjugates
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Purification
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Antigen-specific affinity chromatography followed by absorption with dimethyl arginine
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Conjugation
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Reductive amination; HRP/Ab ratio: 2:1
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Specificity
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The antibody recognizes proteins with methylated arginine residues. It does not cross react with acetylated proteins or mono- and dimethylated proteins.
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Species Tested
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Human, mouse and pig
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Applications
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ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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Product Specific References
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1. Mol Microbiol. 2007. 65: 590-606. doi:10.1111/j.1365-2958.2007.05831.x
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