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Methyl (mono) Arginine Antibody
产品编号: ICP0801
  • 100.00µg
    ¥2875.00
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详细介绍

Methyl (mono) Arginine Antibody

Catalog#

Product Description

ICP0801

This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues.

Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1µg/mL) by free mMeR (IC50=40-50 ng/mL)



Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)

Fluorescent Staining of MMRU cells

Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
NG-mono-methyl arginine-KLH conjugates
Purification
Purified by antigen-specific affinity chromatography
Specificity

The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues. It does not cross react with either asymmetric or symmetric dimethyl arginine.

Species Tested
Pan-specific regardless of species.
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

Product Specific References

  1. 1. J. Proteome Res. 2013. 12 (9): 3884-3899, doi:10.1021/pr400556c
  2. 2. Proteomics. 2013. 13 (7): 1059-1064. doi:10.1002/pmic.201200477
  3. 3. Mol. Microbiol. 2007. 65 (3): 590-606, doi:10.1111/j.1365-2958.2007.05831.x
  4. 4. Proteomics. 2015. 15 (18): 3209-3218. doi 10.1002/pmic.201500075
  5. 5. Proteomics. 2016. 16 (3): 465-476. doi: 10.1002/pmic.201400564
  6. 6. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015




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