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Dimethyl (sym) Arginine Antibody
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Catalog# Product Description
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ICP0811 This antibody is raised from rabbits that were immunized with NG,NG′-Dimethyl-L-arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG′-Dimethyl-L-arginine antigen Agarose column. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (symmetric dimethylation) residues.
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Western blot of human MMRU cells with Diemthyl (sym) Arginine antibodies (ICP0811) and Actin antibody
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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NG,NG′-Dimethyl-L-arginine-KLH conjugates
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Purification
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Antigen-specific immunoaffinity purification with NG,NG′-Dimethyl-L-arginine and absorbed with NG-mono-methyl arginine.
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Specificity
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The antibody recognizes proteins with dimethylated arginine residues. It does not cross react with acetylated proteins or mono-methylated proteins.
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Applications
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ELISA (1:2000), WB (1:1000), IP (5 µg/mg protein sample), IF (5 µg/mL), IHC (5 µg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.
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Product Specific References |
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