Dimethyl Arginine Antibody

Catalog #

Product Description

ICP0810

This antibody was raised from rabbits that were immunized with N^G,N^G-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the N^G,N^G-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.

Western blot of human MMRU cells with ICP0810 (A,B) plus competitive inhibition with free dimethyl arginine (C,D)

Antigen Structure

IF Staining of MMRU cells

Species

Rabbit

Formulation

250μg/mL antibody stored in PBS, 50% glycerol

Immunogen

N^G,N^G-dimethyl arginine-KLH conjugates

Purification

Antigen-specific immunoaffinity purification with N^G,N^G-dimethyl arginine and adsorbed with N^G-monomethyl arginine

Specificity

The antibody recognizes proteins with dimethylated arginine residues. It does not cross-react with acetylated proteins or monomethylated proteins.

Species Tested

Human, mouse and pig

Applications

ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)

Scientific Description

Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

1. J. Proteome Res. 2013. 12 (9): 3884-3899. doi:10.1021/pr400556c
2. Mol. Microbiol. 2007. 65 (3): 590-606, doi:10.1111/j.1365-2958.2007.05831.x
3. Proteomics. 2016. 16 (3): 465-476. doi: 10.1002/pmic.201400564
4. Proteomics. 2013. 13 (7): 1059-1064. doi: 10.1002/pmic.201200477
5. Proteomics. 2015. 15 (18): 3209-3218. doi: 10.1002/pmic.201500075
6. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015

联系我们

电话：0771-2310996 传真：0771-2310995 E-mail ： 735565094@qq.com 技术支持E-mail： immunechem@gmail.com 备案号：桂ICP备09007987号地址：广西南宁市高新技术开发区科园大道68号东盟慧谷11栋203室 530007

网站支持：飞数互联

抗体抗原

标记抗体抗原

工业原料抗体抗原

测试盒

Dimethyl Arginine Antibody

Catalog # Product Description	ICP0810 This antibody was raised from rabbits that were immunized with N^G,N^G-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the N^G,N^G-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.	Western blot of human MMRU cells with ICP0810 (A,B) plus competitive inhibition with free dimethyl arginine (C,D) Antigen Structure IF Staining of MMRU cells
Species	Rabbit
Formulation	250μg/mL antibody stored in PBS, 50% glycerol
Immunogen	N^G,N^G-dimethyl arginine-KLH conjugates
Purification	Antigen-specific immunoaffinity purification with N^G,N^G-dimethyl arginine and adsorbed with N^G-monomethyl arginine
Specificity	The antibody recognizes proteins with dimethylated arginine residues. It does not cross-react with acetylated proteins or monomethylated proteins.
Species Tested	Human, mouse and pig
Applications	ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description	Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
Product Specific References	1. *J. Proteome Res.* 2013. 12 (9): 3884-3899. doi:10.1021/pr400556c 2. *Mol. Microbiol. 2007. 65 (3): 590-606, doi:10.1111/j.1365-2958.2007.05831.x 3. Proteomics. 2016. 16 (3): 465-476. doi: 10.1002/pmic.201400564 4. Proteomics. 2013. 13 (7): 1059-1064. doi: 10.1002/pmic.201200477 5. Proteomics*. 2015. 15 (18): 3209-3218. doi: 10.1002/pmic.201500075 6. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015