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Catalog# Product Description |
ICP0702 The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibodies are conjugated to horse radish peroxidase (HRP). The antibody-HRP conjugates could be utilized for detection, quantization and localization of proteins with Di-methylated lysine residues. |
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Western blot analysis of the di-methylated proteins from swine spleen cell lysate with (B) and without (A) the presence of methylated BSA, using anti-di-methyl lysine antibody HRP conjugates (anti-MeK HRP, ICP0702)
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Species
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Rabbit
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Formulation
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250 µg/mL in Tris-phosphate buffer with 1% BSA and 50% glycerol
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Immunogen
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Di-methylated KLH conjugates
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Purification
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The antibody is immunoaffinity purified with epsilon N-Di-methyl lysine on agarose.
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Conjugation
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Reductive amination with antibody/HRP molar ratio = 1:1
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Specificity
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The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested.
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Applications
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ELISA, WB
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Scientific Description
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Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example, calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.[1]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.
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Product Specific References |
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