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Catalog # Product Description |
ICP0701 The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with Di-methylated lysine residues. |
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Western blot analysis of the di-methylated proteins from swine spleen cells lysate (lanes 4-5) and acid-extraction histones. (Lanes 2-3) with rabbit anti-di-methyl lysine antibodies (anti-MeK, ICP0701).
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Species
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Rabbit
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Formulation
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250 µg/mL in Tris-phosphate buffer, 50% glycerol
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Immunogen
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Di-methylated KLH conjugates
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Purification
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The antibody is immunoaffinity purified with epsilon N-Di-methyl lysine on agarose.
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Specificity
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The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested.
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Applications
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ELISA, WB, IP
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Scientific Description
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Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.[1]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.
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References
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1. Mol. BioSyst., 2013,9, 2231-2247
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