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首页 >> 产品 >> 抗体抗原 >> 蛋白甲基化 >> Trimethyl Lysine Antibody
Trimethyl Lysine Antibody
产品编号: ICP0601
  • 100.00µg
    ¥2875.00
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详细介绍

Trimethyl Lysine Antibody

Catalog #

Product Description

ICP0601

This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum using chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.

Immunohistochemistry of the human tissue with ICP0601
Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with ICP0601.                                            A. Primary antibody                      B. Primary antibody plus 50mg/mL of methylated protein BSA (ICP0504)
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose

Specificity
Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species   Tested
Human, rat, mouse, bovine and pig
Applications
ELISA (1:2000); WB (1:2000); IP (5 µg/mg protein sample); IF (5 µg/mL or 1:100)
Scientific Description
In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe2+, and oxygen.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is three years from date of shipping if stored properly.

Product Specific References

1. J. Bacteriol. 2012. 194 (23): 6410-6418.

doi: 10.1128/JB.01379-12

2. J Biol Chem. 2001. 36 (276): 33512-33517.

doi:10.1074/jbc.M105929200

3. Biochem. Biophys. Res. Commun. 2014. 451 (2): 229-234.

doi:10.1016/j.bbrc.2014.07.110

4. Proteomics. 2015. 15 (13): 2166-2176. doi: 10.1002/pmic.201400521

5. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E

6. Sci Rep. 2016. 6: 35432. 1-24. doi: 10.1038/srep35432


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