Trimethyl Lysine Antibody

Catalog #

Product Description

ICP0601

This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum using chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.

Immunohistochemistry of the human tissue with ICP0601

Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with ICP0601. A. Primary antibody B. Primary antibody plus 50mg/mL of methylated protein BSA (ICP0504)

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Methylated KLH conjugates

Purification

N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose

Specificity

Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.

Species Tested

Human, rat, mouse, bovine and pig

Applications

ELISA (1:2000); WB (1:2000); IP (5 µg/mg protein sample); IF (5 µg/mL or 1:100)

Scientific Description

In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe²⁺, and oxygen.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.

Product Specific References

1. J. Bacteriol. 2012. 194 (23): 6410-6418.

doi: 10.1128/JB.01379-12

2. J Biol Chem. 2001. 36 (276): 33512-33517.

doi:10.1074/jbc.M105929200

3. Biochem. Biophys. Res. Commun. 2014. 451 (2): 229-234.

doi:10.1016/j.bbrc.2014.07.110

4. Proteomics. 2015. 15 (13): 2166-2176. doi: 10.1002/pmic.201400521

5. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E

6. Sci Rep. 2016. 6: 35432. 1-24. doi: 10.1038/srep35432

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Trimethyl Lysine Antibody

Catalog # Product Description	ICP0601 This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum using chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.	Immunohistochemistry of the human tissue with ICP0601 Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with ICP0601. A. Primary antibody B. Primary antibody plus 50mg/mL of methylated protein BSA (ICP0504)
Species	Rabbit
Formulation	250 µg/mL in PBS, 50% glycerol
Immunogen	Methylated KLH conjugates
Purification	N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose

Specificity	Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species Tested	Human, rat, mouse, bovine and pig
Applications	ELISA (1:2000); WB (1:2000); IP (5 µg/mg protein sample); IF (5 µg/mL or 1:100)
Scientific Description	In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe²⁺, and oxygen.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.
Product Specific References	1. J. Bacteriol. 2012. 194 (23): 6410-6418. doi: 10.1128/JB.01379-12 2. J Biol Chem. 2001. 36 (276): 33512-33517. doi:10.1074/jbc.M105929200 3. Biochem. Biophys. Res. Commun. 2014. 451 (2): 229-234. doi:10.1016/j.bbrc.2014.07.110 4. Proteomics. 2015. 15 (13): 2166-2176. doi: 10.1002/pmic.201400521 5. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E 6. Sci Rep. 2016. 6: 35432. 1-24. doi: 10.1038/srep35432