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Catalog # Product Description |
ICP0603 This rabbit polyclonal antibody is raised with a chemically methylated protein antigen. The specific anti-tri-methyl-lysine antibody was affinity purified with trimethylated lysine antibodies and affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The purified anti-trimethyl-lysine antibody was labeled with FITC. The antibody could be utilized for detection and localization of proteins with N-trimethylated lysine residues. |
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Immunofluorescent staining of paraformaldehyde fixed human melanoma cells (MMRU) with ICP0603 (5 µg/mL in PBSt, 3% BSA, r.t, 30 min)
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Formulation
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250 µg/mL in PBS with 50% glycerol
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Immunogen
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Methylated KLH conjugates
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Purification
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The antibody was purified on N (epsilon) tri-methyl lysine agarose and absorbed by mono- and di-methyl lysine.
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Conjugation
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Flourescein 5(6) isothionate; FITC/Ab molar ratio - >20:1
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Specificity
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The antibody recognizes proteins with trimethylation on their lysine residues (N-epsilon). There are no cross reactions with acetylated proteins or mono- and di-methylated proteins.
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Species Tested
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Human and mouse
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Applications
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IF
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Scientific Description
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Trimethylation of the epsilon amino group of lysine residues in proteins is one of the most important events of post-translational modification.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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