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Catalog# Product Description |
ICP0518
The affinity purified rabbit polyclonal anti-Mono-methylated lysine antibody was developed using a technique unique to ImmuneChem. The Mono-methylated lysine antibody was affinity purified using N-Mono-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibody could be utilized for detection, quantization and isolation of proteins with Mono-methylated lysine residues. |
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Western blot analysis of the Mono-methylated histone from melanoma cells with anti-mono-Methylated lysine (anti-mMeK, (ICP0401) Histones were isolated with cold 0.1M HCl extraction followed by this method. A: Blot with 0.5 µg/mL of anti-mMeK (ICP0401) in PBSt + methylated BSA (ICP0504) 100 µg/mL B: Blot with 0.5 µg/mL of anti-MeK (ICP0401) in PBSt
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Species |
Rabbit |
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Formulation |
250 µg/mL in PBS, 50% glycerol. |
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Immunogen |
>10 mg/mL; antibody was covalently linked to CL-4B sepharose |
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Specificity |
The antibody recognizes proteins or peptides Mono-methylated on the lysine residues. The antibody is slightly (30%) cross-reacted with dimethyllysine, not cross-reacted with trimethylysine and acetyllysine. |
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Purification |
The antibody was immunoaffinity purified with epsilon N-mono-methyl lysine on agarose. |
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Applications |
ELISA: 0.25-0.5 ug/mL WB: 0.25-0.5 ug/mL IHC: 2-5 ug/mL IP: 10-20 ug/mg crude protein |
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Scientific Description |
MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that Mono-methylation of lysine plays an important role in gene silencing.
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Storage & Stability |
Product is stable for several weeks at room temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
