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Product Description
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The carboxymethyl lysine (CML) antibody is developed using carboxymethylated KLH as immunogen, and affinity purified with CML-agarose. This antibody can be utilized for the detection, quantification and isolation of proteins/peptides with carboxymethyl lysine residues.
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Western Blot of CML-modified proteins from HUVEC treated with glyoxal (GO) for 48 h. The sample were enriched and blotted with the Carboxymethyl Lysine Antibody (CN1040)
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Carboxymethylated KLH conjugates
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Purification
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Immunoaffinity purified with (ε-N)-carboxymethyl lysine-agarose
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Specificity
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The antibody recognizes proteins or peptides with carboxylmethylated lysine residues. No cross reation with acetylated or methylated proteins/peptides
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Applications
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ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IF; IHC
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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| References |
BioRxiv 2020.10.16.342311 (2020) doi:10.1101/2020.10.16.342311.
Nature Communications. 2021. 12(1):6743. doi: 10.1038/s41467-021-26982-6
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