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Catalog# Product Description |
ICP0206 Affinity purified rabbit polyclonal anti-p42 MAPK antibodies are developed with the C-terminal peptide of human MAPK (ERK2). The purified anti-ERK2 antibody was conjugated to peroxidase (HRP) via reductive amination. The conjugated antibody will facilitate the assay by avoiding background due to the use of secondary antibody conjugates. |
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Western-blot analysis of HeLa cell lysate with anti-ERK2 HRP (ICP0206)
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Formulation
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250 µg/mL in peroxidase stabilizer
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Immunogen
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Human p42 ERK2 C-terminal peptide-KLH conjugates
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Purification
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Affinity purified with p42 ERK2 C-terminal peptide on agarose
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Conjugation
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Reductive amination, HRP/Ab ratio - 5:1
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Specificity
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The antibody recognizes human, rat, bovine, mouse and rabbit p42 ERK1. It does not cross-react with p44 ERK1.
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Applications
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ELISA (0.5 µg/mL); WB (0.5 - 1 µg/mL)
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Storage & Stability
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Product is stable at 4°C for 12 months. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is six months from date of shipping if stored properly.
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