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Catalog# Product Description |
ICP1828 The anti-Interferon Alpha2 antibody from clone 7F4 was raised with recombinant human interferon Alpha2. The antibody was affinity purified from the cell culture medium using anti-mouse IgG Agarose (ICP9705) affinity chromatography. The purified antibody conjugated to peroxidase (HRP), could be utilized for antigen-capture sandwich ELISA when paired with immobilized antibody from another clone 4F6. The antibody is highly sensitive for ELISA development |
Detection curve for human interferon a2 (pg/mL). Immobilized antibody: ICP1818 7F4 (1 µg/mL)
Detetion antibody HRP conjugates: ICP1826 4F6 (1 µg/mL)
OD development: TMB at room temperature for 30 min, stop with 1M H2SO4, read at 450nm
Sensitivity: approximately 10-30 pg/mL
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Species
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Mouse
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Clone
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7F4
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Immunogen
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Recombinant human interferon α2
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Purification
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The antibody was purified from the cell culture media with anti-mouse IgG specific antibody Agarose
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Conjugation
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reductive amination. Ab/HRP ratio=1/2
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Formulation
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250 μg/mL antibody stored in PBS, 50% glycerol or lyophilized powder
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Storage & Stability
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Product is stable for 30 days at room temperature. For extended storage, store product at –20ºC. Expiration date is 12 months from date of shipping if properly stored.
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Specificity
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The antibody recognizes human interferon α2. Cross reactivity to other species not tested
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Applications
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Interferon-capture sandwich ELISA, by pairing with primary antibody from clone 4F6 (ICP1816)
IP
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