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首页 >> 产品 >> 标记抗体抗原 >> Agarose偶联填料 >> Mouse IgG Antibody, Agarose
Mouse IgG Antibody, Agarose
产品编号: ICP9705
  • 40.00mg
    ¥3500.00
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详细介绍

Mouse IgG Antibody, Agarose

Catalog#

Product Description

ICP9705

Monoclonal antibody production using cell culture systems sometimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, both ascites and the supernatant of the cell culture media, without pulling-down bovine IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity (> 10 mg/mL).

Formulation
1 mL beads in 2 mL 50% glycerol
Binding Capacity   >10 mg/mL of mouse IgG
Applications
Affinity chromatography ; Immunoprecipitation
Ligand Immobilized 40 mg anti-mouse IgG/mL
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

 
Product Specific References
  1. 1. JTH. https://doi.org/10.1111/jth.14522
 
Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.
Fraction 1: 0.8 mg/mL
Fraction 2: 2.5 mg/mL
IgG Std: 0.31 - 5 mg/mL
 

 
 

 



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