扫一扫，添加微信号
|
Catalog# Product Description |
ICP9705 Monoclonal antibody production using cell culture systems sometimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, both ascites and the supernatant of the cell culture media, without pulling-down bovine IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity (> 10 mg/mL). |
|
|
Formulation
|
1 mL beads in 2 mL 50% glycerol
|
|
| Binding Capacity | >10 mg/mL of mouse IgG | |
|
Applications
|
Affinity chromatography ; Immunoprecipitation
|
|
| Ligand Immobilized | 40 mg anti-mouse IgG/mL | |
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
|
|
| Product Specific References | ||
|
Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.
Fraction 1: 0.8 mg/mL
Fraction 2: 2.5 mg/mL
IgG Std: 0.31 - 5 mg/mL
|
|
|
