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Catalog# Product Description |
ICP0612 d-Biotin is a natural vitamin that binds to avidin strongly and irreversibly. This antibody was developed and affinity purified using d-biotin as an antigen. The antibody binds biotin strongly but irreversibly. This antibody has been used for direct competitive ELISA for quantization of d-biotin and western-blot analysis of biotinylated antibodies and proteins. |
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Species
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Rabbit
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Formulation
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1 mg/mL in stabilizing buffer
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Immunogen
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d-Biotin-KLH conjugates
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Purification
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Affinity purified
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Conjugation
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Reductive amination; HRP/Ab molar ratio - 10:1
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Specificity
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The antibody reacts with free biotin and biotinylated antibodies and proteins.
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Applications
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Quantitative ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at –20ºC. Avoid cycles of freezing and thawing. Expiration date is six months from date of shipping if properly stored.
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