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Catalog # Product Description |
ICP1327 This product is designed for as the paired antigen of the anti-thyroxine (T4). T4 is linked to Bovine serum albumin (BSA) via its amine group to the free thiol of cysteine residues. It is useful as immobilized antigen for anti-T4 antibody for assay in both ELISA and gold colloid strip test. |
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Structure of the Product
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Formulation
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500 µg/mL in TBS
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Conjugation
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Iodoacetyl-T4 linked to BSA via thiol ether.
T4/BSA ratio: 4:1
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ELISA Titer
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OD50 ELISA titer for T4-BSA is approximately 0.05 µg/mL when 0.1 µg/mL of anti-T4 was used for the test.
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Applications
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ELISA: suggested immobilized concentration: 0.05-0.2 µg/mL
Gold colloid: to be optimized by users
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at –20ºC. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if properly stored.
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