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Catalog# Product Description |
ICP9920 Affinity purified rabbit polyclonal anti-p90 RSK1 antibodies were developed with the C-terminal peptide of human RSK1 (p90) and were conjugated to horse-radish peroxidase (HRP), through reductive amination.The conjugate has been used for one-step Immunoblotting of crude cell lysates and immunoprecipitated RSK1. The use of HRP conjugates reduce the time of blotting and background, particularly the interference of IgG bands resulting from IP. |
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One-step Immunoblotting with cross-antibodies of the immunoprecipitated kinases.
A and C: IP with anti-p90 RSK1
B and D: IP with anti-p70 S6K
A and B: IB with anti-p70 S6K HRP conjugate, ICP9917
C and D: IB with anti-RSK1 HRP conjugate, ICP9920
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Species
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Bovine (Brain from Canadian source).
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Formulation
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250 µg/mL in HRP stabilizing buffer
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Storage & Stability
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Store product at –20 oC. One year from date of shipping
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Immunogen
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Human p90 RSK1 C-terminal peptide (C-LAQRRVRKLPSTTLKLH) KLH conjugates
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Purification
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The antibody was affinity purified with C-LAQRRVRKLPSTT on agarose.
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Specificity
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The antibody recognizes 90 kD RSK1 and does not cross-react with p70 S6K.
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Conjugation
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Reductive amination with a 2/1 molar ratio of HRP/antibody.
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Applications
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ELISA, WB.
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Scientific Description
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Rsk1 is a member of a family of 90kDa ribosomal S6 kinases. It is activated in response to mitogenic stimuli such as extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK, in vitro and in vivo, via phosphorylation and plays an important role in transcription regulation.
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References
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Richards, S. A. et al. Curr. Biol. 1999, 9, 15, 810-820.
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