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Acetyl Lysine Antibody, FITC
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Catalog# Product Description |
ICP0385 The affinity purified rabbit polyclonal anti-acetylated lysine antibody was developed using a unique technique that utilizes acetylated KLH as the immunogen and acetylated lysine-specific antibodies. It was specifically purified with immobilized acetylated lysine on agarose. The purified antibody is conjugated to fluorescein isothiocyanate. |
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Immunoflourescent staining of the TSA-treated and non-treated melanoma cells.
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Species
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Rabbit
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Formulation
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250 µg/mL antibody in PBS, pH 7, with 50% glycerol
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Immunogen
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Acetylated KLH conjugates
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Purification
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The antibody was purified on acetyl-lysine agarose.
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Conjugation
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FITC/Ab ratio - 10:1, followed by G25 filtration
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Applications
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For direct immunofluorescence experiments
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Scientific Description
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Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. [1]Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. [3]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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Product Specific References |
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