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Acetyl Lysine Antibody
产品编号: ICP0380
  • 100.00µg
    ￥3050.00
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详细介绍

Acetyl Lysine Antibody

Catalog#

Product Description

ICP0380

This pan-specific antibody is affinity purified using acetyl-lysine affinity chromatography. It recognizes proteins with acetylated lysine residues. The product has been utilized for proteomic studies of acetylated proteins and immunoaffinity chromatography separation and isolation of acetylated proteins and peptides from protease-digesting proteins of whole cells.

A           B
Western blot analysis of the acetylated protein profile in HeLa cell lysate with anti-acetyl lysine antibodies (ICP0380, lane A) and with additional acetylated BSA    (10 ug/mL) in the primary antibody (lane B).
Species
Rabbit
Formulation
250 μg/mL antibody in PBS, 50% glycerol
Immunogen
Acetylated KLH conjugates
Purification
The antibody was purified on acetyl-lysine agarose.
Specificity
The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP. There are no cross-reaction to non-acetylated proteins.
Applications
ELISA; WB (1:1000); IP; Immunofluorescence;  Immunochemistry
Scientific Description
DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g., transcription factors) and histones may be involved. Acetylation of these proteins may result in signal transduction within the chromatic domains.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
Product Specific References
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