HDAC-8 Antibody

Catalog #

Product Description

CN0830

The affinity purified rabbit polyclonal anti-HDAC8 antibody is developed using the C-terminal peptide of human histone deacetylase 8. It could be used for detection and isolation of HDAC-8.

Western blot analysis of HeLa cell lysate 10 µg (left) and 30 µg (right) with CN0830.

Species

Rabbit

Formulation

0.25 mg/mL in TBS, 50% glycerol, 0.01% NaN₃

Immunogen

Human HDAC-8 (protein code: NP_060956) C-terminal peptide-KLH conjugate

Purification

The antibody was purified with antigen-specific immunoaffinity chromatography.

Specificity

The antibody recognizes human HDAC-8. It may cross-react with HDAC-8 from other species.

Applications

ELISA; WB; IP

Scientific Description

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from the ε-N-acetyl lysine amino acid on histones or other proteins. Its action is opposite to that of histone acetyltransferase.

HDAC proteins are found in three groups, the first two groups belong to the classical HDACs whose activities are inhibited by trichostatin A (TSA). The third group is a family of NAD+- dependent proteins unaffected by TSA. Homologues to all three groups are found in yeast: reduced potassium dependency 3 (Rpd3) corresponding to class 1, histone deacetylase 1 (hda1) to class 2 and silent information regulator 2 (Sir 2) corresponding to class 3.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

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HDAC-8 Antibody

Catalog # Product Description	CN0830 The affinity purified rabbit polyclonal anti-HDAC8 antibody is developed using the C-terminal peptide of human histone deacetylase 8. It could be used for detection and isolation of HDAC-8.	Western blot analysis of HeLa cell lysate 10 µg (left) and 30 µg (right) with CN0830.
Species	Rabbit
Formulation	0.25 mg/mL in TBS, 50% glycerol, 0.01% NaN₃
Immunogen	Human HDAC-8 (protein code: NP_060956) C-terminal peptide-KLH conjugate
Purification	The antibody was purified with antigen-specific immunoaffinity chromatography.
Specificity	The antibody recognizes human HDAC-8. It may cross-react with HDAC-8 from other species.
Applications	ELISA; WB; IP
Scientific Description	Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from the ε-N-acetyl lysine amino acid on histones or other proteins. Its action is opposite to that of histone acetyltransferase. HDAC proteins are found in three groups, the first two groups belong to the classical HDACs whose activities are inhibited by trichostatin A (TSA). The third group is a family of NAD+- dependent proteins unaffected by TSA. Homologues to all three groups are found in yeast: reduced potassium dependency 3 (Rpd3) corresponding to class 1, histone deacetylase 1 (hda1) to class 2 and silent information regulator 2 (Sir 2) corresponding to class 3.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.