Phospho-S6 Peptide Antibody

Catalog#

Product Description

ICP0110

S6 peptide (KRRRLASLR) is a substrate for S6K and ILK. The affinity purified rabbit polyclonal anti-pS6 antibodies were developed with phosphorylated S6 (KRRRLApSLR) and absorbed with the non-phosphorylated S6 peptide (KRRRLASLR). This antibody has been utilized for the non-radioactive p70 S6K kinase assay.

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Phosphorylated S6 peptide KRRRLApSLR-KLH conjugates

Purification

Affinity purified with the phospho-peptide KRRRLApSLR on agarose and affinity absorbed with the non-phosphorylated S6 peptide

Specificity

The antibody recognizes only the phosphorylated product of S6K. There are no cross reactions with the non-phosphorylated peptide substrate.

Tested

Human breast cancer tissues and mouse cells

Applications

ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive S6K kinase assay

Scientific Description

The S6 substrate peptide (KRRRLASLR) is a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

1. Pept. Res. 1991. 4(3): 158-170.

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Phospho-S6 Peptide Antibody

Catalog# Product Description	ICP0110 S6 peptide (KRRRLASLR) is a substrate for S6K and ILK. The affinity purified rabbit polyclonal anti-pS6 antibodies were developed with phosphorylated S6 (KRRRLApSLR) and absorbed with the non-phosphorylated S6 peptide (KRRRLASLR). This antibody has been utilized for the non-radioactive p70 S6K kinase assay.
Species	Rabbit
Formulation	250 µg/mL in PBS, 50% glycerol
Immunogen	Phosphorylated S6 peptide KRRRLApSLR-KLH conjugates
Purification	Affinity purified with the phospho-peptide KRRRLApSLR on agarose and affinity absorbed with the non-phosphorylated S6 peptide
Specificity	The antibody recognizes only the phosphorylated product of S6K. There are no cross reactions with the non-phosphorylated peptide substrate.
Tested	Human breast cancer tissues and mouse cells
Applications	ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive S6K kinase assay
Scientific Description	The S6 substrate peptide (KRRRLASLR) is a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
Product Specific References	1. Pept. Res. 1991. 4(3): 158-170.