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Catalog# Product Description |
ICP1208 The immunogen is Aflatoxin B1 (AFB1)-BSA conjugates. The antibody was affinity purified with an AFB1-Agarose column and competitively eluted by free AFB1. The antibody was conjugated to peroxidase (HRP) by reductive amination. This anti-AFB1 HRP conjugates could be utilized for detection and quantization of the food-borne mycotoxin AFB1.
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Direct competitive inhibition ELISA using immobilized AFB1-BSA (ICP1203, 0.1 mg/mL) and anti-AFB1 HRP (0.1 µg/mL), 50% inhibition occurred at 0.5 ng/mL of free AFB1 in non-optimized conditions.
ELISA Titration of anti-AFB1 HRP (µg/mL). |
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| Species |
Mouse
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| Formulation |
Lyophilized powder from 100 mL PBS solution
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| Immunogen |
AFB1 o-methylcarboxylate conjugated to BSA.
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Purification |
Antigen-specific affinity. |
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| Specificity |
The antibody recognizes Aflatoxin B1 specifically, cross reactivity to other aflatoxins not tested yet.
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| Applications |
Direct ELISA
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| Scientific Description |
Aflatoxin B1 is a food borne mycotoxin which is carcinogenic to both humans and animals. Contamination with AFB1 in food may be harmful to humans and animals.
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| Storage & Stability |
Product is stable for 3 months at 4°C or 6 months at -20°C. Expiration date is 6 months from date of shipping if stored below -20°C.
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