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Catalog# Product Description |
ICP1204 This mouse monoclonal anti-AFB1 antibody was raised using AFB1-KLH conjugates as the immunogen. This antibody can be utilized for the development of competitive ELISA with paired antigen AFB1-BSA (ICP1203) and AFB1-HRP (ICP1206) conjugates for the detection and quantization of the food-borne mycotoxin AFB1. |
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Direct competitive inhibition ELISA using immobilized anti-AFB1 (ICP1204, 0.5 µg/µL) and AFB1 HRP (0.25 µg/mL), 50% inhibition occurred at 0.1 ng/mL free AFB1 in a non-optimized condition.
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Structure of the immunogen and paired antigen
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Species
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Mouse
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Formulation
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1 mg/mL in PBS.
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Paired Antigen
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AFB1 o-methylcarboxylate conjugated to BSA and HRP
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Purification
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Zinc sulfate precipitation
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Specificity
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The antibody recognizes aflatoxin B1 specifically; cross reactivity to other aflatoxins not tested yet.
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Applications
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Direct ELISA, IC50 is approximately 0.1 ng/mL
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Scientific Description
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Aflatoxin B1 is a food borne mycotoxin which is carcinogenic to both humans and animals. Contamination with AFB1 in food may be harmful to humans and animals.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is one year from date of shipping if stored properly.
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