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Catalog # Product Description |
ICP0851 This high affinity His-tag antibody HRP conjugate is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide. The antibody is directly conjugated to peroxidase (HRP) and is useful for detection and localization of His-tag proteins. |
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Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 µL of His-tag protein in lysate (original cell lysate) 3: 10 µL of His-tag protein in lysate (after IP) 4: 10 µL of the His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag (ICP0850) from 2 mL of the original lysate
A: 5 µg of His-tag fusion protein (50 kDa) B: 20 µg His-tag fusion protein (50 kDa)
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Polyhistidine peptide (HHHHHH) KLH conjugates
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The antibody recognizes His-tag proteins.
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Applications
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ELISA (1:2500); WB (0.25-1.0 µg/mL); IHC (5 µg/mL)
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Scientific Description
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Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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