扫一扫，添加微信号
|
Catalog# Product Description |
ICP0115 S6 peptide (KRRRLASLR) is a substrate of S6K and ILK. Affinity purified rabbit polyclonal anti-pS6 antibodies were developed with the phosphorylated S6 (KRRRLApSLR) and absorbed with the non-phosphorylated S6 peptide. This antibody is conjugated to horse-radish peroxidase (HRP) through reductive amination. This antibody has been used for non-radioactive p70 S6K kinase assays. |
|
Species
|
Rabbit
|
|
Formulation
|
250 μg/mL in HRP stabilizing buffer, 50% glycerol
|
|
Immunogen
|
Phosphorylated S6 peptide KRRRLApSLR-KLH conjugates
|
|
Purification
|
Affinity purified with the phospho-peptide KRRRLApSLR on agarose and affinity absorbed with the non-phosphorylated S6 peptide
|
|
Conjugation
|
Reductive amination with 2/1 molar ratio of HRP/antibody
|
|
Specificity
|
The antibody recognizes only the phosphorylated product of S6K. There are no cross reactions with the non-phosphorylated peptide substrate.
|
|
Tested
|
Human breast cancer tissues and mouse cells
|
|
Applications
|
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive S6K kinase assay
|
|
Scientific Description
|
The S6 substrate peptide (KRRRLASLR) is a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.
|
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.
|
|
Product Specific References
|
1. Pept. Res. 1991. 4(3): 158-170.
|
