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Catalog# Product Description |
ICP0180 Crebtide (KRREILSRRPSYR) is a substrate peptide derived from the protein Creb which is widely utilized as a synthetic substrate for the PKA and PKC kinase families. Affinity purified rabbit polyclonal anti-pCrebtide is developed with phosphorylated Crebtide (KRREILSRRPpSYR) and absorbed with non-phosphorylated Crebtide. |
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Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A: 0.0 ng B: 3 ng C: 6 ng D: 12.5 ng E: 25 ng F: 50 ng
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A:0.0 ng B:4 ng C:15 ng D:60 ng E:250 ng.
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Species
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Rabbit
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Formulation
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250 µg/mL in TBS, 0.1% NaN3
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Immunogen
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Phospho-Crebtide (KRREILSRRPpSYR)-KLH
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Purification
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The antibody was affinity purified with KRREILSRRPpSYR on agarose followed by affinity desorption with non-phosphorylated Crebtide.
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Specificity
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The antibody recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross-reactions with the non-phosphorylated Crebtide.
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Applications
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ELISA; WB; Non-radioactive PKA and PKC kinase assay
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Scientific Description
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p33 (ING1) is a new growth inhibitor candidate for a tumour suppressor gene. p33 (ING1) cooperates with p53 and down-regulates cell growth by activating transcription from the p21/WAF1 promoter although there is no sequence similarity between p33 (ING1) and p53.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.
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Product Specific References
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