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| Catalog# ICP1948 |
| Product Description |
Papain is conjugated to agarose bead via amine linkage. The product can be repetitively used as an immobilized papain for various proteolysis applications. It can efficiently hydrolyze antibodies into Fc and Fab fragments.
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Sample Data
Figure1 Preparation of Goat anti-Mouse IgG Fab
One gram of purified Goat anti-Mouse IgG (lane 1) was loaded onto the Papain Agarose column (10 mL) at a speed of 0.5 mL/min. The flow-through (lane 2) was subsequently affinity bound to a Mouse IgG Agarose column, and the pure Fab fragment (lane 3) was then purified. |
| Papain Immobilized |
60-300 units/mL of papain (30 mg/mL) is covalently linked to Sepharose CL-4B
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| Formulation |
5 mL of wet agarose beads suspended with 5 mL of glycerol
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| Applications |
Enzymatic hydrolysis of proteins Preparation of antibody Fc/Fab fragments
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| Storage & Stability |
Product is stable for 4 weeks at ambient temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if properly stored.
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