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Papain Agarose
产品编号: ICP1948
  • 10ml
    ¥2500.00
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详细介绍

Papain Agarose

Catalog#            ICP1948
Product Description

Papain is conjugated to agarose bead via amine linkage. The product can be repetitively used as an immobilized papain for various proteolysis applications. It can efficiently hydrolyze antibodies into Fc and Fab fragments.

 

 

 

 Sample Data

 

Figure1 Preparation of Goat anti-Mouse IgG Fab

 

One gram of purified Goat anti-Mouse IgG (lane 1) was loaded onto the Papain Agarose column (10 mL) at a speed of 0.5 mL/min. The flow-through (lane 2) was subsequently affinity bound to a Mouse IgG Agarose column, and the pure Fab fragment (lane 3) was then purified. 

Papain Immobilized

60-300 units/mL of papain (30 mg/mL) is covalently linked to Sepharose CL-4B

 

Formulation
5 mL of wet agarose beads suspended with 5 mL of glycerol

 

Applications

Enzymatic hydrolysis of proteins

Preparation of antibody Fc/Fab fragments

 

Storage & Stability

Product is stable for 4 weeks at ambient temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if properly stored.

 

 

 


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