• 咨询电话:0771-2310996
  • 扫一扫,添加微信号

首页 >> 产品 >> 测试盒 >> 蛋白激酶活性试剂盒 >> Broad Spectrum Serine/Threonine Kinase Assay Kit
Broad Spectrum Serine/Threonine Kinase Assay Kit
产品编号: ICP0248
  • 96 Assays/Kit
    ¥3500.00
相关产品
详细介绍

Broad Spectrum Serine/Threonine Kinase Assay Kit

Catalog#

Product Description

ICP0248

This product is designed for testing the activity of a variety of serine/theonine kinases (STK) (see Table 1) and screening the inhibitors/activators of corespondinging kinases. The assay kit is non-radioactive but colorimetric.

The principle of the assay:
The kinase substrates is immobilized onto the 96-well plate. The kinases use ATP to phosphorylate the substrate on the plate. The phosphorylated product is detected by the phospho-substrate specific antibodies. See Figure1 for the principle illustration.
Formulation
1x96 assays or 5x96 assays
Applications
Assay for purified or partially purified serine/threonine kinases listed in Table 1
Assay for inhibitors of the serine/threonine kinases listed in Table 1
Storage & Stability
Product is stable for 30 days at room remperature. 6 months at 4°C.
Kit Compoentes
1. 96-well Substrate Microtiter Plate (Part#: KA101)
2. Phosphospecific Substrate Antibody(Part#: KA102)
3. Anti-Rabbit IgG: HRP Conjugate(Part#: KA103)
4. Antibody Dilution Buffer (Part#:KA104)
5. Kinase Assay Dilution Buffer (Part#:KA105)
6. ATP(Part#: KA106)
7. 20X Wash Buffer (Part#:KA107)
8. TMB Substrate (Part#:KA108)
9. Stop Solution (Part#:KA109)

Figure 1   Assay Principle

Table 1 Activity of STKs Tested by the Assay Kit


联系我们
电    话:0771-2310996 传    真:0771-2310995 E-mail : 735565094@qq.com 技术支持E-mail: immunechem@gmail.com 地    址:广西南宁市高新技术开发区科园大道68号东盟慧谷11栋203室 530007 备案号:桂ICP备09007987号