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Catalog# Product Description |
ICP0751 This high affinity anti-HA antibody is developed using the HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. This antibody is conjugated to horse radish peroxidase (HRP) through reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. |
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Western blot analysis of the HA-tag protein (50 kDa) expressed cell lysates with HA antibody HRP conjugates (ICP0751) 1: Marker 2: 25 µL/lane 3: 15 µL/lane 4: 5 µL/lane 5: 2 µL/lane
A B C
Western blot analysis of the positive cell lysates with expression of HA-tag protein.
A: Marker B: 1 µL/lane C: 10 µL/lane
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Species
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Rabbit
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Formulation
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250 µg/mL antibody in peroxidase buffer
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Immunogen
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Human influenza virus hemaglutinin (HA) peptide, (YPYDVPDYA) KLH conjugates
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The antibody recognizes proteins fused with the (HA) peptide, YPYDVPDYA.
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Conjugation
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Reductive amination; HRP/Ab molar ratio - 10:1
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Applications
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ELISA; WB; IP
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Scientific Description
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Human influenza virus hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of HA-fusion proteins.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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