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Colog#
Product Description
ICP0902
This high affinity anti-Flg (DYKDDDDK) antibody is developed using the Flg peptide (DYKDDDDK) conjugated to KLH and affinity-purified with the DYKDDDDK peptide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody is useful for detection, isolation and localization of DYKDDDDK-tag proteins.
Western blot analysis of the cell extract with Flg-tag protein expression cell lysate ( 1 mg/mL)
lane 2 = 25 µL; lane 3 = 15 µL; lane 4 = 10 µL cell lysate.
Western blot analysis of the cell extract with Flg-tag protein expression (lane 7 = 25 µg; lane 8 = 5 µg; lane 9 = 10 µg cell lysate)
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Flg peptide (DYKDDDDK) KLH conjugates
Conjugation
Reductive amination; HRP/Ab ratio - 2:1
Specificity
The antibody recognizes DYKDDDDK-tag proteins.
Applications
ELISA (1:2500); WB (1 µg/mL)
Scientific Description
DYKDDDDK peptide (Flag-tag) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of the DYKDDDDK-fusion proteins.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
