Chloramphenicol Antibody

Catalog#

Product Description

CN1025

This antibody was developed using chloramphenicol-KLH conjugates as the immunogen and purified with chloramphenicol-agarose affinity chromatography. The antibody could be utilized for isolation, detection and quantization of chloramphenicol.

Direct competitive inhibition ELISA using immobilized anti-chloramphenicol (3 µg/mL), and chloramphenicol-labelled HRP (100 ng/mL). 50% inhibition occurred at 50 ng/mL of free chloramphenicol under non-optimized conditions.

Species

Rabbit

Formulation

250 µg/mL in PBS with 50% glycerol

Immunogen

Chloramphenicol-KLH conjugates

Purification

Antigen-specific affinity chromatography

Specificity

The antibody recognizes free chloramphenicol.

Applications

ELISA; Affinity chromatography

Scientific Description

Chloramphenicol is a bacteriostatic antimicrobial agent originally derived from the bacterium Streptomyces venezuelae, isolated by David Gottlieb, and introduced into clinical practice in 1949. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms and is still very widely used in low income countries because it is exceedingly inexpensive. Detection and quantization of chloramphenicol in food (feed) is becoming common due to the issue of antibiotic abuse.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

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Chloramphenicol Antibody

Catalog# Product Description	CN1025 This antibody was developed using chloramphenicol-KLH conjugates as the immunogen and purified with chloramphenicol-agarose affinity chromatography. The antibody could be utilized for isolation, detection and quantization of chloramphenicol.	Direct competitive inhibition ELISA using immobilized anti-chloramphenicol (3 µg/mL), and chloramphenicol-labelled HRP (100 ng/mL). 50% inhibition occurred at 50 ng/mL of free chloramphenicol under non-optimized conditions.
Species	Rabbit
Formulation	250 µg/mL in PBS with 50% glycerol
Immunogen	Chloramphenicol-KLH conjugates
Purification	Antigen-specific affinity chromatography
Specificity	The antibody recognizes free chloramphenicol.
Applications	ELISA; Affinity chromatography
Scientific Description	Chloramphenicol is a bacteriostatic antimicrobial agent originally derived from the bacterium Streptomyces venezuelae, isolated by David Gottlieb, and introduced into clinical practice in 1949. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms and is still very widely used in low income countries because it is exceedingly inexpensive. Detection and quantization of chloramphenicol in food (feed) is becoming common due to the issue of antibiotic abuse.
Storage & Stability	Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.