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Catalog# Product Description |
ICP1202 The immunogen is Aflatoxin B1 (AFB1)-BSA conjugates. The antibody was affinity purified with an AFB1-Agarose column and competitively eluted by free AFB1. The antibody was conjugated to peroxidase (HRP) by reductive amination. This anti-AFB1 HRP conjugates could be utilized for detection and quantization of the food-borne mycotoxin AFB1. |
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Direct competitive inhibition ELISA using immobilized AFB1-BSA (ICP1203, 0.1 ug/uL) and anti-AFB1 HRP (0.1 µg/µL), 50% inhibition occurred at 0.5 ng/mL of free AFB1 under a non-optimized condition.
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Species
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Rabbit
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Formulation
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0.5 mg/mL in PBS, 1% BSA, 0.01% proclin in 50% Glycerol or lyophilized powder.
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Immunogen
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AFB1 o-methylcarboxylate conjugated to BSA
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Purification
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Antigen-specific affinity chromatography and free antigen competitive elution system.
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Specificity
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The antibody recognizes Aflatoxin B1 specifically; cross reactivity to other aflatoxins not tested yet.
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Applications
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Direct ELISA
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Scientific Description
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Aflatoxin B1 is a food borne mycotoxin which is carcinogenic to both humans and animals. Contamination with AFB1 in food may be harmful to humans and animals.
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Storage & Stability
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Product is stable for 3 months at 4°C or 6 months at -20°C. Expiration date is 6 months from date of shipping if stored properly.
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